Its ubiquitous gene deletion in mice leads to embryonic lethality associated with severe cardiovascular defects, including aberrant organization of vessel networks, impaired coverage of vessels by mural cells, and abnormal heart development.
As duplicated reads are of no added value and duplication artefacts can impair coverage-based quality validation, they should be removed prior to the assembly.
Local PDGF unavailability has proved to impair the coverage of newly formed vessels with mural cells and local pericytes [ 56].
While a pre-digestion of immobilized DNA with all marking enzymes is sufficient to completely eliminate redundancy, it impairs the coverage.
Moreover, we found that Uncl-Sema3E (and p61) impaired pericyte coverage of blood vessels (Fig 5E and Supporting Information Fig 6B), leading to reduced vessel functionality and perfusion (Fig 5F) as well as increased vessel permeability (Supporting Information Fig 6C).
Impaired perivascular cell coverage causes vascular malfunctions such as leakage and hyperplasia [ 28].
In an important new finding, we uncovered confusion between routine retinal photography at optometry practices during eye examinations and DRS. While optometry photography may represent an important safeguard for non-attenders, it could impair more comprehensive coverage.
In real world clinical settings, however, concerns have arisen regarding adverse effects of DES in some patients, including incomplete neointimal coverage, impaired endothelial cell function, thrombosis, hypersensitivity reactions, and incomplete stent apposition [ 4– 7].
