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In order to investigate if impaired formation of viral clusters resulted in enhanced activation of the IFN cascade, the transcript levels of type I (IFN-α and IFN-β) and type III (IL-29) IFN, and of the IFN-stimulated gene viperin, were quantified at 8 and 16 hpi.
Knockdown of EphA2 in malignant melanoma cells impaired formation of the VM network [29].
RNAi ablation of SYT/L alone or both isoforms markedly impaired formation of stress fibers and focal adhesions but did not affect formation of cortical actin bundles.
An impaired formation of gap junctions in rl−/− mice could therefore also underlie the observed phenotype.
Interestingly, each of the four mutations significantly impaired formation of the ∼110-kDa parkin complex (Fig. 9A and B).
Myoblasts isolated from Bmal1−/− mice exhibited impaired formation of organized myotubes and a considerably lower percentage of MHC-positive myonuclei.
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Bisphosphonates delay osteoclastic bone resorption but may also impair formation of new bone.
10.7554/eLife.01310.004 Figure 2. Inhibition of Zap70 activity impairs formation of both the cSMAC and pSMAC.
This in turn implies that disruption of one bridge in the Ig-like domain impairs formation of the other.
Additional considerations are that any FIBTEM amplitude below 2 mm is assigned a value of zero, and that fibrinolysis may potentially impair formation of the fibrin-based clot.
Moreover, suppression of PODXL in MDA-MB-231 cells profoundly impairs formation of primary tumors and secondary metastasis in xenografted mice.
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