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EndoS activity affects the functionality of opsonizing IgG by decreased binding to Fc-receptors on a monocyte-like cell line and impaired classical complement activation in vitro [26].
Complement component C3 is an important constituent of the complement system, of which activation and deposition indicate complement activity.
It has been suggested that infection with human papillomavirus (HPV) and/or Epstein Barr virus (EBV), which are prevalent in certain squamous cell carcinomas 90, may alter complement activity 88, given that viral proteins are known to impair the immune response 91.
Reconstitution of complement activation with rFicolin-3 allowed full restoration of complement activity.
The assessment of in vitro complement activity was analysed using a complement screening kit (Wieslab COMP300).
Vlam, L. et al. Complement activity is associated with disease severity in multifocal motor neuropathy.
To exclude that complement activity played a role, whole blood circulation experiments were also conducted at 10 U/ml of soluble heparin, a concentration which inhibited complement function.
Total complement activity, complement 3 (C3), and soluble complement C5b-9 levels were measured in the sera of rats using ELISA-based methods.
Although systemic complement activity was reduced in the IVIg-treated rats, cerebral complement and complement inhibitor factor levels were mostly comparable among treated and non-treated groups.
Complement activity was determined using a hemolytic assay.
Complement activity was normal.
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