We have established that this inhibitory phenomenon is both time- and concentration-dependent, as shorter 4 h soaks in 0.1 mg/ml dsRNA had no negative impact on M. incognita J2 stage worms, yet a 10-fold increase in concentration to 1 mg/ml for the same 4 h time period had an even greater qualitative and quantitative impact on worm phenotype and motility.
On the other hand, no impact on worm motor activity was observed in the groups treated with no lethal concentrations of all active compounds (Table 2).
Mebendazole or RXR agonist treatment showed no significant impact on worm vitality at any of the concentrations tested, using the MTT assay.
Of note, one study did not illustrate a detectable impact of worm carriage on a rodent model of cerebral malaria, suggesting that various experimental host parasite combinations can yield different results [23].
He considered the impact of worm propagation delay and bandwidth constraint on its spread but ignored the impact of human actions.
A sensitivity analysis was conducted where each parameter at each temperature was increased by up to a factor of 10 and the impact on mean worm burden per individual and prevalence measured (note that the results for these are qualitatively similar, so only the results for mean worm burden are presented here).
To assess the impact of selecting sheep for lower WEC (i.e. an increase in the frequency of the A allele), we simulate a range of sheep flocks with different frequencies for A and deduce the impact on the worm population of selecting sheep for lower WEC by interpolation between the different flocks.
Our findings imply that the impact of metformin on worm lifespan reflects the sum of indirect, E. coli-mediated life-extending effects and direct life-shortening effects.
Also, the texture and hardness of the waste may have had an impact on the worms' reproduction.
We first generated pch-2 over-expressing C. elegans lines by a microinjection method (Supplement Fig.1), and studied the functional impact of pch-2 on worm longevity.
