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"As long as all those energies and resources are used in these incremental steps beyond the cold war, you won't get the kind of serious, entrepreneurial pattern of experiment, re-experiment, doctrinal innovation and organizational creativity -- all those things that have an impact on transformation," Mr. Hillen said.
Several parameters concerning the co-cultivation were tested in order to assess their impact on transformation frequency.
Briefly, 400 ml C. thermocellum was grown to an OD between 0.8 and 1.0, centrifuged without measures to exclude oxygen, since washing the cells in the presence of O2 seemed to have no impact on transformation efficiency (unpublished observations), at room temperature in a Beckman Coulter Avanti J-25 centrifuge with a JA-10 rotor at 5000 × g, and the supernatant was removed.
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We demonstrate how spatial restriction on population growth and DNA diffusion in the environment affect the impact of transformation on adaptation.
We demonstrate how the impact of transformation on adaptation varies in different parts of a growing population, depending on DNA diffusion, bacterial migration and the source of free DNA in the environment.
We examined the impact of transformation on adaptation in the course of colonization of a new environment.
Since spatial restriction on population growth limits the availability of free DNA to bacteria, we expect the impact of transformation on population dynamics to be different in spatially restricted compared to the planktonic populations.
Meanwhile, the addition of acetosyringone to induce the transformation process was found not to have much impact on the transformation of sago palm cells (data not shown) despite the reports that stated that the addition of acetosyringone is recommended in the transformation of monocotyledonous plant [ 19, 38– 40].
We investigated the impacts of transformation on recruitment into the canopy tree guild by measuring seedling establishment across the fence line contrasts.
A special panel will be created ostensibly to examine the court's judgments and their impact on social transformation.
It is known that factors which require much amount of effort may not have that much impact on MSEs' transformation or vice versa.
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