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Cost, for example, is still an important limitation, and has a crucial impact on replicates.
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In the Charité laboratory variable noise induced by operating conditions had a similar impact on total noise as replicate noise.
The fold change cut-offs selected were necessarily lower than for the transcriptomics approach due to iTRAQ ratio compression, hence their impact on false discovery rates across biological replicates was assessed and found to be low for all runs [Additional file 2].
PSG manager Carlo Ancelotti ushered David Beckham off the bench in the closing stages, but the former England captain could not replicate Messi's impact on the game as the visitors struggled to force their way back onto the front foot.
We show evidence that effect size, and therefore sensitivity, has a significant impact on likelihood of local-eQTL being replicated by the cis-eQTL study.
The aim of returning the culture to the initial conditions was to illustrate that the method of culturing had no impact on the results, and, to provide replicate data to prove this point.
Replicate had a significant impact on HPG development (F5,108 = 6.24, p < 0.001).
And they also raise a challenge: for these programs to have significant impact on our financial lives, they need to be replicated on a national scale.
Thus, as a routine, we included activated charcoal in the microplate agar assay and used the resulting CARA to study the impact of TB-active agents on replicating and nonreplicating M. tuberculosis bacilli.
If minimal number of replicates is not used, outlier removal has a slight impact on data quality (data not shown).
It is a trend replicated in most debates about the EU's impact on policy and public services.
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