This mode of recognition provides an elegant immunological solution for tight binding to an aliphatic, lipid-like ligand with a small head group lacking typical haptenic features, such as aromaticity or charge, which are often incorporated into hapten design to generate high-affinity antibodies.
Another immunological solution for specific antibody binding to dense clusters has been reported for the 2G12 IgG, which is highly specific for the complex oligomannose glycans that decorate the "silent face" HIV-1 gp120 [58].
Unfortunately, we were not able to obtain a complete data set for fungal sensitization, in part due to a lack of immunological testing solutions.
After dissolving the biotinylated tracer in enzyme immunological assay buffer, the solution was used within a week or was alternatively frozen in smaller aliquots until use.
Furthermore, minimal immunological reactions were observed when the collagen solution was injected in mice treated with or without adjuvant therapy, without significant dilution of the sera [ 50].
The recovered solutions were subjected to biochemical and immunological analysis.
In this paper, we present the solutions developed to analyze the tumor immunological microenvironment in humans including database, analytical tools, and tools for visualization.
One part of organ samples was cut off and fixed in 4% formaldehyde solution, and the other parts were used for immunological assays.
Membranes were stained for protein content with Ponceau S solution (Sigma-Aldrich, St . Louis MO), and destained for immunological detection according to the manufactuer's protocol.
To prevent immunological rejection and unexpected infectious diseases in regenerative therapy, one of the solutions is to use the patient's own cells.
