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During the prophase Myc immunofluorescence appeared distributed in subnuclear domains in a number of intensely fluorescent spots which tended to aggregate, during the subsequent prometaphase the immunofluorescence intensity decreased throughout the nucleus with the exception of the spindle poles, and finally faded away during the anaphase.
In cells treated with lytic peptide, the immunofluorescence intensity of lytic peptide increased in a concentration and treatment time-dependent manner.
This paper presents a miniaturized and high sensitive cytometer using a microfluidic chip for evaluating the radiation dose by detecting the mean immunofluorescence intensity of γ-H2AX γ-H2AX
We measured immunofluorescence intensity in random fields.
Immunofluorescence intensity was measured in the plane with maximum fluorescence.
AC3 immunofluorescence intensity density was measured using ImageJ software (NIH, Bethesda, MD).
The fluorescence intensity was normalized by the AMPAR immunofluorescence intensity of EGFP-overexpressing neurons.
For quantification of immunofluorescence intensity, two-tailed paired Student's t test with two-sample unequal variance was used.
Both AC3 immunofluorescence intensity density, OMP and β-tubulin immunopositive cell numbers were normalized to saline-treated group.
For each condition, the reduction of E-cadherin immunofluorescence intensity by TGFβ was expressed as a percent of the E-cadherin immunofluorescence level in the respective basal (TGFβ-untreated) cells.
Quantification of immunofluorescence intensity was performed using IMAGE-J software (version 1.37a, National Institutes of Health, USA) where the integrated density of pixels (sum of the gray values of each pixel in determined area) of the given image was measured.
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