cAMP levels were quantitatively measured using the Cyclic AMP Enzyme Immunoassay kit (Alfa Aesar) according to the manufacturer's instructions.
Nitrite/Nitrate concentrations were assayed with a Nitrite/Nitrate immunoassay kit (R&D System) using Griess reagents following the instructions.
Novel comparison of CDT isoforms as determined by CE with an FDA-approved immunoassay kit.
Melatonin levels were determined taking all the necessary care to prevent light degradation by using the melatonin enzyme immunoassay kit (IBL Hamburg, Germany).
These results were in statistical agreement with those obtained by commercial assay of enzyme-linked immunoassay kit (ELISA).
The CGRP contents of the samples were measured by enzyme-linked immunoassay kit (Bertin Pharma, France).
Plasma D-dimer levels were determined using a quantitative immunoassay kit at enrollment, before each treatment, and at the time of trial completion or disease progression.
Amounts of the cysLTs C4, D4 and E4 (as determined with the enzyme immunoassay kit specified in the following paragraph) were below the detection limit.
Salivary cortisol levels were measured in batches (by year) using an enzyme immunoassay kit according to the kit instructions (Salimetrics, State College, PA, USA).
Cortisol was measured in plasma samples using an enzyme immunoassay kit (Diagnostic Systems Laboratories, Webster, TX).
Plasma corticosterone concentration was determined with an enzyme immunoassay kit (Assay Designs, Ann Harbor, MI, USA).
