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Goat was immunized using primary intramuscular injection of 200 µg refolded recombinant antigen dissolved in 1 ml of NaCl 0.9% and emulsified at 1∶1 in 1 ml of Alum.
The mice were immunized using 0.2 ml for each group [ 7] (Table 2).
Balb/c mice were immunized using rat FRβ gene-transfected B300-19 cells.
Unadjusted risk ratio (RR) and 95% confidence interval (CI) were estimated for the study outcome (DPT-3/Hepatitis B fully immunized) using bivariate Poisson regression [ 19, 20].
That is why we have come together in the Global Alliance for Vaccines and Immunizations (GAVI), a coalition dedicated to ensuing that all the world's children are immunized, using every effective vaccine available.
Rabbits were immunized using a standard immunization program at Agrisera (Vännäs, Sweden).
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Seventeen weeks after the 2nd booster the mice were re-immunized using the same protein concentration and sera were collected 10 days later.
Seventeen weeks later, the mice were re-immunized using aliquots of the same r-GV preparations and sera collected at 10 days and again at 43 weeks post re-immunization.
To demonstrate that vaccination initiated in young 3xTg-AD mice without pre-existing AD-like pathology is beneficial as they age to 18±0.5 mo old, we measured spatial learning and memory in control (naïve or immunized with plasmid encoding MDC fused with irrelevant antigen) and immunized animals using the Morris Water Maze (MWM) [36].
To this end, we performed a comparative gene transcription analysis of livers from mock-immunized and WED-immunized zebrafish using RNA-seq technology to investigate their differential transcriptsomic profile.
To evaluate the impact of the administration of Palivizumab on the risk of being hospitalized, we performed additional Poisson models that estimated IRRs for immunized compared to non-immunized infants using two different approaches of selecting specific at risk time periods among those who received prophylaxis.
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