The induction of IDO converts the tumor-draining LN from an immunizing into a tolerizing milieu.
The recombinant baculovirus expressing ROBO1 was immunized directly into gp64 transgenic mice.
10 isolated lymphocytes from IsdB immunized or BSA immunized mice were transferred into each recipient mouse via the tail vein.
Mice (9 10 months old) were immunized by injection into the flank with Aβ1-42 (100 µg) or proteolipid protein (PLP 139-151 (400 µg; GenScript Corp., Piscataway, NJ, USA) emulsified in complete Freund's adjuvant (CFA) H37Ra (Difco, Detroit, MI, USA).
Xiao et al.[ 4] found that transfer of anti-MPO IgG from MPO-deficient mice immunized with mouse MPO into wild-type mice led to pauci-immune vasculitis.
For these experiments we isolated CD45.1 memory cells or effector cells from 6 week S. mansoni immunized mice and transferred into CD45.2 S. mansoni immunized mice and again used flow cytometry to determine the level of Notch receptor expression on the expanded donor cell population (1d e).
10 cells were adoptively transferred intravenously into TN immunized mice.
This phenomenon was not observed when Foxp3+ cells were transferred into mice immunized with OVA alone, which suggests that the inflammatory conditions in the gut favor Tregs conversion into IL-17-producing cells.
DBA/1 mice, 7 weeks of age, were intradermally immunized at several sites into the base of the tail with 200 μg bovine type 2 collagen (CII) dissolved in 100 μl of 0.05 M acetic acid and mixed with an equal volume of complete Freund's adjuvant (Sigma, Oakville, ON, Canada).
(I ) Pre-Tfh 4 days after transfer of 1 × 10 OT-II T cells into CD45.2 C57BL/6 hosts immunized with OVA I.P. followed by CD80/86 blocking antibodies I.V. 64 hr following immunization.
