Sentence examples for immunized cells from inspiring English sources

Experimental data unambiguously indicate the existence of intercellular signals in immunized cells, which are spread all over the tissues making them immunized [29],[29].

The in vitro immunized cells were infected with EBV by virus-containing supernatant derived from the EBV-producing marmoset cell line B95-8 (AmeriCulture Collection, ATCCion, ATCRLCRL 1612; kindly provided by Dr. L.-F. Sheu, Tri-Service General HospiTaipeiaipei).

For secondary immunization, 3 × 107 primary-immunized cells were mixed with the peptide in a flask that had been immobilized overnight with 5 mg/ml of CD40L (CD154; eBioscience) together with 1 × 107 QYIKANSKFIGITEL (Fine Research Biochem -stimulated CD4+ T cells and 5 ng/ml rh IL-15 (eBioscience).

In the scrambled siRNA control sample, immunized target cells showed effector cell dose-dependent cell death, whereas pretreatment with TGF- β1 for 1 h blocked cell death.

In the absence of TGF- β1 pretreatment, an increase in effector (OT-1 mouse-derived CD8+ T cell -to-target ratio incell -to-targetdiated cell -to-target immunized MC38 cells.

To test the impact of IL-10 signaling in antigen-specific T-cell proliferative response in immunized mice, cells recovered as described above were cultured in 96-well U-bottom plates in the presence of different concentrations of CII for 3 days, followed by pulsing with 1 μCi of [H]thymidine per well for the last 18 hours, and proliferation was measured as radioactivity incorporated (in cpm).

Samples of pooled immunized donor cells used for adoptive transfer were tested for their proliferative capacity by 3H-thymidine incorporation after stimulation with either immunizing or irrelevant antigen.

In addition, immune T cells from immunized mice could transfer partial protection against a C. trachomatis genital challenge to naı̈ve mice.

In agreement with the high production of cytokines related to Th1 skewed immunity, spleen cells of immunized mice showed higher mRNA levels for T-bet and lower for GATA-3, compared with the levels of the two control groups (Fig. 2A and 2B).

We also did not detect any effects of SDF-1 on the proliferative response of IIC-immunized T cells to IIC stimulation.

T cell proliferation assays of immunized lymph node cells were set up as described above.