Exact(1)
No significant antibody or IFN-g responses were seen at baseline or after the priming immunizations (data not shown).
Similar(59)
High levels of PA-specific titers remained in the serum of C3d-immunized mice for over 60 days post-secondary immunization (data not shown).
Similar laser adjuvant effects were also observed when endotoxin-removed OVA was used for immunization (data not shown), ruling out that the immune-enhancing effect was attributed in part to endotoxin contamination.
Preliminary testing of viral titers in lung, nose, kidney, and brain at day 6 showed significant differences in the lungs and noses between A/NP+A/M immunized mice and controls, suggesting some impact of the immunization (data not shown).
Briefly, all mice immunized with INA developed high titer anti-INA Abs 8 9 weeks after immunization (data not shown).
No natural killer cells were observed anywhere in the brain at 14 to 19 days post-immunization (data not shown).
The secondary expansion of tetramer+ CD8+ T cells was not observed in the absence of anti-CD40 during booster immunization (data not shown).
Despite the observed differences in immunogenicity, both VN1203ΔNS1 and VN1203ΔNS1-K58I viruses completely prevented the replication of the homologous challenge virus VN1203wtNS in the lungs of 3/5 and 4/5 animals, respectively, taken 3 days post immunization, (data not shown) showing no statistically significant difference.
In the same way, no significant difference in Th1, Th2 or Th17 related cytokine secretion was seen after GM and non-GM maize protein extract restimulation of splenocytes from the treated animals, whatever the route and extract used for immunization (data not shown).
Similar results were observed in DBA/1 mice upon immunization (data not shown).
However, we did not detect a difference in the number of Tregs in the brains of ONX 0914 treated mice 19 days after MOG35 55 immunization (data not shown).
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