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However, when nasal immunization is performed in rabbits or non-human primates, animals with a nasal cavity structure/anatomy that closely resembles the human nasal cavity, nasal immunization is often not as effective as immunization delivered with a needle.
Briefly, when immunization is performed with a modified self-protein like EPO, cross-reactive neutralizing antibodies are secreted and deplete the circulating protein.
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If MVA immunization was performed three days after infection, all intranasally immunized mice died with the same kinetics as unvaccinated control mice, but still none of the intravenously vaccinated mice succumbed (Fig. 1B).
Considering that the pancreatic cancer is a very lethal disease and panc02 cells could easily develop tumors in the C57BL/6 mice, the therapeutic immunization was performed 4 days after the inoculation and the mice were immunized 3 times.
The immunization was performed by intra-muscular injection of pcDNA3-PRA, and the antiserum was detected by ELISA.
Using native KLH no response was found, whereas 1 / 64,000 serum dilutions gave very high values in ELISA procedures when immunization was performed using the hyper-succinylated KLH.
Immunization was performed twice at 10-day intervals.
Primary immunization was performed using a group of 8 mice by s.c. injections.
The first immunization was performed using Complete Freund's Adjuvant (CFA) (Sigma-Aldrich, F5581, St . Louis USA).
Since the immunization was performed using bacterially produced rmPrP23-231 whish is not glycosylated, the paratopes are unlikely to be directed against a glycan epitope.
Immunization was performed as reported in [16] and MSCs (1×106 cells) were injected intravenously on day 18 and 24, otherwise indicated.
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