Your English writing platform
Discover LudwigExact(3)
Three days after the final immunization, cells from the lymph nodes of the immunized mice were fused with P3-U1 myelomayeloma cells in the presence of 50% (w/v) polyethylene glycol (PEG4000) (Wako).
(F ) WT and IL-6RαT-KO mice were immunized with 2W peptide and LPS emulsified in IFA and 7 days after immunization cells isolated from the draining lymph nodes were stained with 2W I-Ab tetramer to determine percentages of CD4+ Foxp3+ 2W I-Ab+ cells.
Twenty-four hours after adult immunization, cells were detected in the draining LN associated with the fluorochrome-labeled formulation.
Similar(57)
Mouse immunization, cell fusion and HAT selection were carried out as previously described [ 13].
Furthermore, except for specific immunization, cell death in the perivascular tissues was also unaffected by host treatment.
Six days after immunization, cell suspensions from inguinal and periaortic lymph nodes (LN) of 5 mice were prepared in RPMI 1640 (GIBCO) supplemented with 10 mM HEPES, 50 μM 2-Mercaptoethanol, 216 mg L-glutamine/L and 5 % FCS (GIBCO).
Three weeks after the initial immunization, demyelination and immune cell infiltration in the spinal cord were examined.
Four days after the booster immunization, spleen cells of the immunized mouse were fused with mouse myeloma Sp2/0 cells using PEG 1500 as a fusion agent (PEG/DMSO solution, HybriMax, Sigma-Aldrich Co).
Eight days after immunization, donor cells were analysed for TFH cell differentiation.
Three days after the final immunization, LN cells were fused with P3U1 myeloma cells.
At the high dose of immunization (5×106 cells per mouse), both CMT.TAP1/B7.1-immunized and CMT.B7.1/p-immunized mice were well protected from live tumor attack (Fig. 2C left-panel).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com