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Specific activity of xylanase produced under immobilized condition was found 2.8 times higher than that produced under free cells.
She was hospitalized in an immobilized condition due to both the crural ulcerations and the existing heart-failure state (NYHA III-IV) having pleural and pericardial effusions, atrial fibrillation and weight loss of 30 kg over the past year.
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Figure 3 shows CV under bare and immobilized conditions.
Eleven carbon, 11 organic nitrogen and seven inorganic nitrogen sources were screened by two-level Plackett Burman design for maximum alkaline protease production by using optimized immobilized conditions.
Repeated fed batch mode of operation, using optimized immobilized conditions, resulted in continuous operation for 12 cycles without disintegration of beads.
Previous experiments and results in this paper indicated that 150 µmol m−2 s−1 were stressful for chlamydomonas cells in immobilized conditions (Rea G, unpublished data; Fig. 4).
The use of operant behavioral tasks that utilize the head-immobilized condition in rodents have some advantages over tasks that occur under freely moving conditions by limiting the range of possible non task-relevant behaviors and distractors.
Crucial parameters are the design of stable and reproducible surfaces which maintain biological activity of immobilized proteins, and immobilization conditions (buffer, pH, concentration).
Immobilization conditions and characterization of the immobilized enzyme were investigated.
Hence, the patient was admitted to a dermatologic department in an immobilized, weak condition having heart failure due to hypertensive and ischemic heart disease (left ventricular ejection fraction: 30%%, NYHA III-IV).
We have not used competition curves to derive dissociation constants because we measured the ability of free ligand to block binding of the Smo CRD to bead immobilized ligand a condition in which the two ligands are clearly chemically different (hence violating a key assumption when using competition to derive dissociation constants).
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