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The immobilization yields were calculated as the ratio of immobilized protein to the protein subjected to immobilization.
The optimum temperature time combinations for the highest immobilization yields were determined for both methods.
Immobilization yields (specific activity ratio of entrapped lipase to free lipase) of cellulose, cellulose carrageenan, cellulose chitosan, cellulose agarose, and cellulose agar bead were 35.0, 9.6, 39.7, 41.4, and 52.6%, respectively.
The immobilization on LH-HA and BB-A finished at 36 h and the immobilization yields (more than 90%) of these two supports were almost the same as ZH-HA, but the activity recovery was only 38.9% and 28.2%, respectively.
Immobilization yields and recovered activities depended on the respective immobilization protocol (Table 3).
When glutaraldehyde concentration is raised up to 5%, immobilization yields are almost comparable at RT for 4 h or overnight at 4°C (see R5 and R6).
Similar(52)
The behavior of high immobilization yield and immobilized enzyme activity may be explained by the fact that the strategy of immobilization was appropriate and the amine groups in chitosan have been highly activated with glutaraldehyde, and amine groups of the proteins were easy to link with glutaraldehyde-activated chitosan.
The immobilization yield was determined by dividing immobilized beads specific activity by total enzyme specific activity used for immobilization.
The surface modification resulting in the highest enzyme immobilization yield, was used to covalently immobilize the trypsin on the inside wall of a fused-silica capillary.
Inulinase was immobilized on chitosan particles with 66.4 ± 0.9%% immobilization yield and 36.4 ± 0.4%% activity yield.
Three commercially available polymers (Sepabeads® EC-EP, Sepabeads® EC-HA and Purolite® A-109) were tested for potential application as supports for covalent immobilization of lipase from Candida rugosa by analyzing some critical properties of immobilized enzymes such as enzyme loading, activity and activity immobilization yield.
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