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The immobilization yield was determined by dividing immobilized beads specific activity by total enzyme specific activity used for immobilization.
The surface modification resulting in the highest enzyme immobilization yield, was used to covalently immobilize the trypsin on the inside wall of a fused-silica capillary.
Inulinase was immobilized on chitosan particles with 66.4 ± 0.9%% immobilization yield and 36.4 ± 0.4%% activity yield.
For a given amount of dry support, results showed that the immobilization yield decreased with the amount of initial laccase activity while the immobilized activity (IU/g) approached a constant value.
The immobilization yield reached about 71%.
In the present studies, activity yield was lesser compared to immobilization yield.
Open image in new window Fig. 1 Effect of laccase protein concentration on the immobilization yield.
The high sensitivity was related to the surface coverage and hence the immobilization yield.
The values of APTT and TT increased with increasing immobilization yield of heparin.
Optimized immobilization variables enhanced the immobilization yield (IY) from 27.9 to 79.9% (2.9-fold).
Immobilization yield was optimized using a statistical method, central composite design.
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