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To demonstrate the utility of this approach more generally for optimized enzyme immobilization, two 20-mer peptides, YHNNPGFRVMQQNKLHHGSC (referred to as YHNN) and QYHHFMNLKRQGRAQAYGSC (referred to as QYHH) were selected from a microarray of 10,000 peptides based on their ability to bind β-galactosidase (β-gal) and optimize its surface-immobilized activity (Table S1 in File S1).
In order to obtain stable, efficient and economic enzyme preparations suitable for potential biotechnological applications, in this work three hydrophobic and porous materials were tested as supports for lipase immobilization: two polypropylene matrices (Accurel EP100 and MP1000) and a diatomaceous silica (Celite®545).
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Twenty patients aged <40 years with primary anterior shoulder dislocation without associated fractures were randomized to post-reduction external rotation <span class="lh">immobilization (nine patients) or internal rotation (11 patients).
The difference between these three systems lies in the immobilization of two or three enzymes into a single or in different sol gel slides.
Ankle fractures surgically treated normally need stabilization of the ankle mortise using either staples or a screw [ 8, 9, 11] and immobilization for six to eight weeks after surgery in a plaster cast or in an orthosis [ 51].
Electrodeposited electrodes were further functionalized to carry out covalent immobilization of two oxygen-reducing multicopper oxidases: laccase and bilirubin oxidase.
A theoretical study is presented dealing with the immobilization of two enzymes into a porous solid support.
To investigate the influence of the electrode structure and the immobilization procedure, two types of enzymatic electrodes have been developed.
The design allows the immobilization of two different receptors on the same biochip with a 5 μl volume/sensor channel.
The oligosaccharide-capped core shell nanoparticles were employed as support for the supramolecular immobilization of two different adamantane-modified enzymes, tyrosinase and xanthine oxidase, through host guest interactions.
A new electrochemical biosensor based on the covalent immobilization of two enzymes on self-assembled monolayer attached to a polycrystalline gold electrode is proposed, experimentally.
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