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The immobilization sequence was paired with 12 complementary bases and immobilized on the gold electrode; the single-stranded detection sequence, rich in G bases, formed a G-quadruplex (G4) structure in the presence of Pb2+.
While the immobilization sequence part remains 'lying' on GO surface.
The adsorbed DNA sequence is specially designed with two parts, including immobilization sequence and probe sequence.
An azide reaction scheme has enabled covalent immobilization onto fluorinated ethylene propylene copolymer but its chemistry is less predictable, requiring detailed characterization by XPS and tapping mode AFM after each step of the immobilization sequence.
Figure 3a shows a control which was just functionalized with the immobilization sequence 4. The other three AFM images show the results of the immobilization of particles via the immobilization linker 5 and cross-linker 6, in which the amount of the cross-linker was varied from 0 eq over 8 to 16 eq (Fig. 3c,d).
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Colonies were randomly selected and robotically processed through RCA of plasmid clones, sequencing reactions using BigDye chemistry (Applied Biosystems), reaction cleanup using solid-phase reversible immobilization, and sequencing using an ABI 3730 XL automated DNA sequencer.
PCR products were purified with solid-phase reversible immobilization, cycle sequenced, and analyzed on an ABI3730 DNA sequencer (Applied Biosystems, Foster City, CA).
Single-molecule spectroscopy may be a suitable tool to study the efficient and sensitive fluorogenic catalytic conversion presented here in greater depth, as using DNA in our catalytic model offers several advantages: facile functionalization, immobilization, and sequence-specific assemblies.
A random selection of these clones was processed for sequencing reads from each end using rolling circle amplification of the plasmids, sequencing reactions using BigDye terminators (ABI), cleanup using solid phase reversible immobilization (SPRI), then sequence determination on an ABI 3730 × l automated DNA sequencer.
HLA: Human leukocyte antigen; NGS: Next generation sequencing; SPRI: Solid phase reversible immobilization; GSSP Groupp specific sequencing primer.
A brief examination of these existing EST sequences indicated that a large proportion of them were trophont only reads, histones, ribosomal proteins, and immobilization antigen-related sequences.
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