We present two surface-immobilization schemes that connect an azurin variant with mutations in the redox cavity to two oligophenylvinyl (OPV) molecular wires, along with an electrochemical study of their electron transfer (ET) properties.
One is the post-immobilization strategy.
Scheme 1 Immobilization of chitosan on glass beads.
Scheme 1 Immobilization of DMC on modified silica.
Open image in new window Scheme 1 The immobilization of glucose oxidase (GOx) on the surface of SWNTs by using enzyme adsorption involves a layer-by-layer technique.
This immobilization process is represented in Scheme 1(a).
This immobilization process is represented in Scheme 1(b).
Fig. 1 The scheme of in situ immobilization of 4-vinylpyridine and styrene copolymer on silica gel surface.
Fig. 1 The scheme of in situ immobilization of poly[N- 4-carboxyphenyl)methacrylamide] on the silica surface.
An azide reaction scheme has enabled covalent immobilization onto fluorinated ethylene propylene copolymer but its chemistry is less predictable, requiring detailed characterization by XPS and tapping mode AFM after each step of the immobilization sequence.
The scheme of in situ immobilization of 4-vinylpyridine and styrene copolymer on silica gel surface is presented in Fig. 1.
