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The newly developed system is a superior design and possesses many advantages, including its simple preparation, low cost, no enzyme immobilization required, high flexibility, and good sensitivity.
Selective spinal clearance (i.e., no immobilization required) guidelines, without regard for the MOI, may be an effective tool for reducing unnecessary immobilization while accurately identifying spinal fractures [2 5].
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Conventional immobilization requires the presence of desirable functional groups within the drug molecule as well as a multistep process to prepare the biotinylated derivatives.
The risk of bleeding increases even further in the inpatient setting because of surgeries, intracranial events, immobilization requiring prophylactic anticoagulation and the increasing use of antiplatelet or other antithrombotic medications often used in the setting of cardiac events [ 11].
In general, the prenylation-based immobilization strategy has several potential biomedical and biotechnology applications where oriented protein immobilization is required, such as protein arrays, and diagnostic applications based on immunoassays, surface plasmon resonance (SPR), or electrochemical methods.
No immobilization was required after surgery, and early weight bearing was encouraged with a rapid return to normal activities.
This result indicates that a high degree of immobilization is required for protein solutions to accurately model tissue.
Because the in vivo immobilized lipase can be prepared easily by simple cultivation and separation of the cells, no additional steps for the purification and stabilization (immobilization) are required and can feasibly be modified by rational de novo design of the enzyme.
Consequently enhanced phosphate mineral precipitation for contaminant immobilization will require the engineered addition and dissemination of phosphate.
However, the carrier and the immobilization procedures require extra expenditure in biocatalyst preparation; in addition, carrier-bound enzymes are usually (much) less active than their native soluble counterparts.
In all, 37.2% (16/42) of patients identified as having a spinal fracture by hospital radiography were either not immobilized or immobilization was not required by the guideline.
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