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Immobilization mixture was prepared in triplicates.
Zeolites were added to the immobilization mixture to improve the standard procedure of cross-linking in glutaraldehyde vapor; the urease adsorption without glutaraldehyde was also described.
Activity of free enzyme in cell extract and supernatant fractions, or in supernatants from the immobilization mixture was analyzed by measuring the release of para-nitrophenol (pNP) from pNP-derivative fatty acid substrates, as a result of enzymatic hydrolysis, measured at λ = 405 nm as previously described [ 21, 38].
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Supernatant samples from immobilization mixtures were taken at different times and their residual lipase activity was assayed as described [ 38].
We show that physiological pressures do not substantially affect response to chemical immobilization with this mixture; however, consideration must be taken for differences that may exist for immobilization of adult males and juveniles.
Surface immobilization of an equimolar mixture of DPPC and l-α-dipalmitoylphosphatidyl glycerol (DPPG) on SPIONs significantly increased colloidal stability of these nanocomposites in physiological buffer systems.
For instance, in the extractive fermentation of cane bagasse, with OAL DAL mixture and cell immobilization, the productivity is increased to 2.5 g/l/h, fivefold higher than that for batch process (Bankar et al. 2012).
The results from this study demonstrate that surface immobilization of an equimolar DPPC/DPPG mixture on SPIONs via high-affinity avidin-biotin interactions increases colloidal stability in the presence of different buffer ions.
We also achieved protein-specific biotinylation and immobilization in a complex protein mixture (E. coli cell lysate) without purifying the precursor protein, which is a considerable advantage of this method for high-throughput protein immobilization on protein chips.
A total of 110 chemical immobilization procedures using Telazol (1 1 mixture of tiletamine and zolazepam) were done on adult female Weddell seals as part of a study on maternal energy expenditure and lactation energetics.
To this end, gold substrates were functionalized by chemisorption of thiolated capture oligomer 4. The DNA-modified gold substrate was used for immobilization of nanoparticles from a mixture, which contained equimolar amounts of D2 AuA and D2 AuB (3 nM, each), in addition to 16 molar equivalents (eq) (0.3 μM) of linker 5 and 16 eq (0.3 μM) of linker 6.
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