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AZD9773 Fab fragments were immobilized using amine-coupling chemistry to achieve a ligand immobilization level of 800RU.
The immobilization level was 3375RU.
The dependence of the immobilization level on the concentration of SPA-Cys was investigated.
It was shown that the introduction of a cysteine residue in the recombinant protein A increases the immobilization level of SPA via a strong interaction of the thiol group with the gold sensor surface.
MASS-1 sensitivity was independent of protein immobilization level and kinetic analysis performed using ultra-low density mAb surfaces allowed characterization of picomolar affinity interactions without mass transport limitation.
The final VEGF immobilization level was approximately 300 RU.
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This yielded immobilization levels of approximately 1,000 (CD44 isoform) and 300 (BSA-peptides) response units, respectively.
An innovative additional pelletization step with only cementitious binder has been performed in order to achieve satisfactory immobilization levels.
The low nanomolar target binding affinity (KD < 7 nM) of AbD15179 reported previously [28] was verified using triplicate injections and two different immobilization levels of CD44v6-10.
Some surface plasmon resonance (SPR) biosensors allow for parallel screening of several running buffer compositions, with a number of different immobilization levels for each buffer.
By examining experimental conditions, including immobilization levels and temperature, for a range of analytes, and by using global analysis of several sample dilutions, conditions that gave the most robust results were identified.
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