Sentence examples for immobilization kit from inspiring English sources
Hybridomas were cultured in DMEM/F-12 supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. Synthetic peptide (CGNQVSKEMKRMGFEDPKDKN) was covalently immobilized to beaded agarose using SulfoLink Immobilization Kit (Pierce Biotechnology).
Npl3 peptides were conjugated to resin using the Carboxylink Immobilization Kit (Pierce).
The antiserum was affinity-purified using the SulfoLink immobilization kit following manufacturer's instructions (Thermo Scientific).
The antibodies were affinity purified with the RBM44 antigens using the AminoLink Plus Immobilization Kit (Pierce, Rockford, IL).
The resultant PLD4 antibody FD130-1 44) was purified by affinity chromatography on an antigenic peptide-coupled SulfoLink column (SulfoLink Immobilization Kit for Peptides, product #44999, Pierce, Rockford, IL) and utilized at a concentration of 1 µg/ml.
The anti-Drp1 and anti-dMfn antisera were purified using the synthetic peptides that elicited these antisera as ligands in affinity chromatography, as described (ThermoScientific SulfoLink Immobilization Kit for Peptides, Thermo Scientific (Waltham, MA) #44999).
Surface plasmon resonance (SPR) measurements were carried out on a BIAcore-T100 instrument using anti-human IgG capture kit or covalent immobilization on CM5 biosensor chips (GE Healthcare).
Using the amine-coupling kit (Biacore) and the Biacore T100 immobilization wizard, purified LDLR ectodomain (His-tagged, R&D Systems) was immobilized to one of the four flow cells of a CM5 sensorchip to a level of approx. 150 RU (resonance units).
A total of 33,600 random plasmid subclones were selected and DNA was purified for sequencing using the Concert96 plasmid purification kit (Invitrogen) or by solid-phase reversible immobilization (SPRI) magnetic beads (SprintPrep; Agencourt Bioscience Corporation).
To isolate total RNA from cell lines of interest we adopted a chemical extraction in combination with a silica-based membrane immobilization by using QIAzol and RNeasy mini and micro kit (QIAGEN, GmbH Hilden, Germany).
LR-PCR products were purified using the AMPure XP Kit (Beckman Coulter) and solid-phase reversible immobilization (SPRI) technology.
