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In order to circumvent this limitation, Wang et al. utilized an immobilization free in vitro SELEX developed by Li and coworkers to select ssDNA MREs specific for four different organophosphorus pesticides, phorate, profenofos, isocarbophos, and omethoate [ 26, 28].
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Immobilization-free DNA-based electrochemical biosensors can provide powerful complementary tools and novel design strategies for traditional electrochemical DNA biosensors.
This review introduces multiple immobilization-free strategies for developing DNA-based homogeneous electrochemical biosensors, aiming at compensating for the deficiencies of heterogeneous modes.
Next, the SAF incorporated SHL assay was extended to fabricate immobilization-free biosensors for rapid sensing of salicylic acid (SA) and β-hydroxybutyrate (β-HB) in whole blood.
In this work, an ultrasensitive immobilization-free photoelectrochemical (PEC) biosensor was successfully developed for the first time based on a novel enzyme-free cascaded quadratic signal amplification strategy.
This work designs a new homogenous electronic monitoring platform for sensitive detection of hepatitis C virus (HCV) on an immobilization-free Ag(I -assisted haI -assistedhairpinthe cytosine–Ag+–cytosine coorDNAathroughemisthe.
Isothermal titration calorimetry (ITC) is one of the most robust label- and immobilization-free techniques used to measure protein – small molecule interactions in drug design for the simultaneous determination of the binding affinity (ΔG) and the enthalpy (ΔH), both of which are important parameters for structure-thermodynamics correlations.
He et al. employed immobilization-free SELEX to identify ssDNA MRE specific for pesticide, acetamiprid.
Nutiu and Li developed a different target immobilization-free SELEX method using a ssDNA library containing a 15-base constant region, sandwiched by two random regions, and finally encompassed by two constant primer hybridization regions at both 3′ and 5′ ends [ 26].
Berezovski and coworkers were the first to use Non-SELEX to identify a high affinity MRE (K d : 0.3 nM) specific for hRas protein [ 22]. Park and coworkers developed an immobilization-free SELEX method based on π- π stacking interaction between DNA and graphene oxide (GO-SELEX).
It should be noted this function appeared to level off and decline at C N larger than 25, approximately the value where microbial nitrogen immobilization limits free soil nitrogen.
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