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Most studies set the period of immobilization between 4 and 8 weeks.
The majority of studies specify this period of immobilization between 4 and 8 weeks [5, 6, 7, 8, 9, 10].
There were no significant differences in either gross mineralization, immobilization, gross nitrification or NO3− immobilization between grazed and ungrazed plots in a paired t-test using all plots (P=0.52, P=0.32, P=0.91 and P=0.93, respectively).
This technique leads to successful single molecule immobilization between electrodes, which is the next step.
First, one would have to examine binding reactions between HSP72 and multiple levels of immobilized peptide to provide assurance that differences in calculated equilibrium dissociation constants reflected differences in peptide sequence and not differences in the level of immobilization between peptides.
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This was a retrospective study of traumatically injured patients based on a clinical clearance spinal immobilization guideline between January 2006 and January 2007.
For example, Warne (1995) examined the toxicity of 24 detergents to the cladoceran Ceriodaphnia cf. dubia and found that toxicity levels (measured as EC50 at 48 h, immobilization) varied between 1.6 and 70.3 ppm.
Epiphyseal separation requires immobilization for between 6 and 9 weeks to achieve satisfactory healing, which is considerably longer than for fractures in which the epiphysis is not involved [24, 31, 35].
Following peptide immobilization, and between each of the subsequent steps, assay plates were washed (4×) with PBST.
The contact time was 7 min, which resulted in immobilization levels between 200 and 1000 response units (RU).
The relationship between immobilization factors and enzyme activity was examined by a series of contour plots.
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