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Fig. 4 Reproducibility of glucose biosensor preparation based on stainless steel electrodes with different types of GOx immobilization: 1 GOx-SME-GA, 2 GOx-GA.
Fig. 3 Calibration curves of glucose conductometric biosensors with different types of GOx immobilization: 1 GOx-SME-GA, 2 GOx-GA.
For enzyme immobilization, 1 ml of lipase solution (1.0 mg ml−1 of lipase in 50 mM, pH 8.0 Tris HCl buffer) was mixed with 18 mg of NPG.
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Vmax for immobilized and free inulinase remained almost unaffected by immobilization (52.6 and 52.0 μmol/ml/min, respectively) revealing that immobilization did not interfere with the reaction velocity.
In contrast, there are many reports in the literature indicating shift in optimum temperature of inulinase towards higher or lower values after immobilization [1, 4, 18, 19].
In previous reports, two patients were treated by open reduction and cast immobilization [1, 2], two by open reduction and percutaneous pinning of the carpal bones and cast immobilization [3, 4], one by open reduction and suture of the anterior capsule and cast immobilization [5], and one by proximal row carpectomy (PRC) (Table 1).
These include fairly complex protocols for covalent immobilization [26, 40].
Surface-functionalized silica particles have found applications in catalysis [7 9], sensors [7, 10], and protein immobilization [11, 12].
Carpobrotus edulis invasion generally increased NH4+ immobilization (INH4, 1.19 4.48 ×), presumably due to a lower N availability for the microbiota.
However, there can also be shift of pH optima after immobilization [10, 16].
This can reduce potentially detrimental consequences of muscle deconditioning secondary to immobilization [15, 16].
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