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After the immobilisation step, chemical structure of surfaces was analyzed by X-ray Photoelectron Spectroscopy. X-ray Photoelectron Spectroscopy
This flow cell was treated in the same way as the other during the immobilisation procedure, but omitting the ligand immobilisation step.
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A versatile one-step immobilisation method was carried out based on the electrochemical polymerisation of a pyrrole monomer functionalised with an Os-complex.
A three-step peptide immobilisation process was then used to immobilise a cysteine-terminated RGD-containing peptides onto the grafted surface.
In this paper, we further exploit this methodology for a convenient one step biomolecule immobilisation onto gold surfaces.
The final step involves the immobilisation of the Au-based colloids onto the desired heterogeneous support, such as carbon, TiO2 or other metal oxide supports [26].
On the basis of a two-step nanoshaving and protein immobilisation process, two different antibodies (mouse IgG and human IgG) were selectively immobilised on shaved nanotrenches [98].
Four hundred nanolitres of this mix was then separated by isoelectric point using the NanoPro1000 (Protein Simple) using the power 1 step separation profile, 100 s immobilisation exposure and 480 min primary incubation.
Surface modification by immobilisation of organic molecules is a very important step and search of new methods is constantly developing [3, 4].
To enable peptide immobilisation, the silicon surface was modified in several consecutive steps to introduce the active amino groups.
However, the crucial polymerisation step requires both time (>10 h) and expertise and the antibody immobilisation is also time-consuming (>18 h).
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