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We also did not find significant differences among the three genotypes in the rotarod or open-field (total distance traveled and total time spent immobile) tests, indicating the absence of salient perturbations in generalized motor activity or coordination.
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In the developing world, the barriers are more fundamental: lack of access to trained eye care professionals, costly and immobile testing equipment, and disorganized and disconnected fulfillment chains.
This could be ruled out for both rocks studied in the present work, since they contained only reducible and oxidizable As, which remained immobile during leaching tests.
The tail-suspension test is used to determine depressive-like characteristics, indicated by the time spent immobile during the test.
During the 6 min test, force of movement or lack thereof was recorded and reflected the time the animal spent immobile during the test.
Using a stopwatch, the time spent immobile over the test period was recorded for each animal.
High-fat fed animals were significantly less immobile during the test (Table 3).
Also, there was a tendency for the C2CR mice to spend more time immobile during the test than did the controls (C2CR mice, 32.9 ± 16.5 s, n = 10; controls, 11.9 ± 7.1 s, n = 9; NS).
To keep the mice immobile throughout this test procedure, they were first anesthetized by intraperitoneal injection of 0.5 ml "Avertin" (2.5 g 2,2,2 tribromoethanol in 5 ml amylene hydrate and 200 ml water).
Because existing antidepressants specifically inhibit the immobile time in these tests, the utility of these experiments is recognized.
The time spent immobile during the swim test did not vary with treatment (Figure S2).
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