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An immersion water bath circulator (Thermomix 1480/Frigomix 1497, Braun, Melsungen, Germany) was connected to the cooling system containing a closed pipe system, installed in moist peat culture substrate to circulate the refrigerating fluid through the moist peat layer.
Refraction occurs at the interfaces between the immersion water, the top glass plate and the sample.
Images were captured every 3 sec using a 20X N.A. 0.7 immersion (water) objective.
We used a 40x 0.8 NA water immersion objective (for its long working distance), and placed a coverslip on top of the intact specimen to provide a barrier between the oil/glycerol mixture and the immersion water column (though in principal, an objective designed for oil/glycerol immersion would improve our results).
Emanating from A at an angle α s with respect to OA, a ray is refracted and crosses the coverslip surfaces at P and B. The refraction angles in the glass coverslip and in the immersion water are α g and α i respectively.
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The church offers full-immersion water baptism, which some people want and others do not.
Measurements were performed in ND96 low Ca2+ (5 mM Hepes, pH 7.5, 96 mM NaCl, 2 mM KCl, 1 m MgCl2) at 18 20°C using a Nikon Eclipse TE 300 microscope with a 20×0.75 NA multi-immersion (water for our experiments) lens.
PC/bare fiber composite annealed at 275 °C for 10 min prior the immersion in water exhibited excellent retention of properties after long-term immersion in hot water.
Immersion in water was used as the control treatment.
Baptism was to be by full immersion in water and was not administered to infants.
Others survive total immersion under water for as long as one or two days.
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