Baseline clinical measurements were collected before and after this first dive (T0 and T1, Fig. 3) in order to have a reference [CTRL] and to measure physiological modifications due to immersion using EAN.
The smear was examined under x100 oil immersion using a light microscope.
The initial weight of each specimen was noted before immersion using an analytical balance (precision: ±0.1 mg).
In all stages, the metaphase spreads were photographed under 1000 X (oil immersion) using a Motic BA400 microscope.
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The low back pain intensity will be assessed after 20 seconds of immersion using the NRS.
Samples were visualised under oil immersion using a Nikon Eclipse TE300 microscope and a 100× objective.
Briefly Drosophila filets were fixed by immersion using 2% glutaraldehyde in 0.1 M cacodylate buffer at pH 7.4 overnight at 4°C.
