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The move comes in the wake of Mayor Rudolph W. Giuliani's push to have the schools institute an English immersion plan similar to one being tried in California.
Specimens were scanned at Nyquist optimal frequency using either a ×40/1.3 oil immersion Plan-Neofluar objective or a ×63/1.4 oil immersion plan apochromate objective.
Images were acquired with 40x/0.75 or 63x/1.4 objective oil immersion plan APO and treated using Axiovision 4.7.2 and Photoshop 5.0 software.
Fluorescence images were obtained using a Zeiss Axiovert200 fluorescence microscope equipped with a 63X oil immersion Plan Apochromat objective and standard filter sets (FITC and rhodamine).
FRET was measured, using acceptor photobleaching [38] with a Zeiss inverted 510 confocal and a 63x NA 1.4 oil immersion Plan Apochromat objective and a software zoom of 2. HaCaT cells were grown on coverslips as described previously.
The sections were examined with a Zeiss LSM 510 META confocal microscope using a ×63/1.4 oil immersion plan apochromate objective, 488 nm (argon ion laser) as excitation light, and a detection of 505 530 nm.
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Imaging of the embryos was done using an inverted LSM 510 Meta laser-scanning microscope with a 40X oil immersion Plan/Fluor (NA = 1.3) objective or 63X oil immersion Plan-Apochromat (NA = 1.4).
Fluorescence images of yeast cells were taken after growth in YPD media overnight at 25°C on a rotator at 20 revolutions per minute with a Zeiss Axiovert 135 TV microscope with 100 x oil immersion Plan-Neofluor objective.
using an inverted LSM710 laser scanning microscope with a 63 × oil immersion Plan-Apochromat (NA = 1.4) objective.
Wild type and transgenic BY-2 cells were imaged using a Leica TCS-SP spectral confocal microscope equipped with an argon ion laser using a 40 × oil immersion Plan-Apo objective (Leica, Wetzlar, Germany).
Confocal microscopy was performed using a LSM 510 laser scanning unit coupled to an inverted microscope with a 63 × 1.4 numerical aperture oil immersion Plan-Apochromat objective (Carl Zeiss).
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