For recording, slices were transferred to an immersion chamber and perfused with a bath solution containing NaCl (126 mM), KCl (3 mM), NaH2PO4 (1.2 mM), NaHCO3 (25 mM), glucose (15 mM), MgSO4 (2 mM) and CaCl2 (2 mM), gassed with carbogen and supplemented with either 20 µM bicuculline methiodide or 20 µM picrotoxin to inhibit GABAA receptors.
Slices were transferred to an immersion recording chamber and perfused (2.5 mL/min) with ACSF.
A trendy technique called cryotherapy offers whole-body immersion in chambers where the temperature can drop to 150 degrees below zero.
In order to record postsynaptic currents in the neurons, the equilibrated hemi-slices were placed in an immersion-type recording chamber.
For recordings slices were transferred to an immersion-type recording chamber (after ≥1 hr incubation for acute slices) and perfused with ACSF comprising (in mM) 127 NaCl, 2.5 KCl, 1.25 NaH25O4, 25 d-glucose, 25 NaHCO3, 4 Cand2, and 4 MgCl2 saturated with 95% O2/5% CO2.
For recordings (1 7 hr after slicing), individual slices were transferred to an immersion-type recording chamber, perfused with ACSF (2 ml min−1) at 24 26 °C.
Brain slices were mounted in the immersion-type recording chamber under the two-photon microscope and continuously perfused with oxygenated artificial cerebrospinal fluid at 30 °C during recordings.
We allowed the slices to recover for at least 60 min and then transferred them to an immersion-type recording chamber perfused at 1 mL/min with ACSF containing 0.1 mM picrotoxin (Sigma-Aldrich, Tokyo, Japan) at room temperature.
The immersion cycle corrosion test chamber is various steel accelerated corrosion test equipment.
Corrosion protection characteristics of the hybrids were examined on low-carbon steel panels by immersion and salt-spray chamber tests.
While Bargemusic supplies a full immersion in Mozart, the chamber society offers a full immersion in Brahms: the three piano trios, ending with the great early and late B minor (Op. 8, revised).
