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Before data collection, the crystals were immersed briefly in a cryoprotectant solution containing additional 20% glycerol.
Before data collection, the crystals were immersed briefly in a cryoprotectant solution, which was the reservoir solution containing additional 15% glycerol.
Sections were immersed briefly in a citrate buffer (0.01 mol li−1 citric acid: pH 6.0) and incubated for two 5-min intervals at 100°C in a microwave oven for antigen retrieval.
Slides coated with worm sections or other parasite stages were immersed briefly in a destaining jar containing 0.2 M Phosphate Buffered Saline (PBS, pH 7.2) to wash off loosely bound materials.
Assembling the Halfcell/Holder: A chlorided silver wire (a silver wire immersed briefly into household bleach, then rinsed) was inserted into the reference barrel to create the connection with the amplifier.
The brain was immersed briefly in oxygenated ice-cold low-calcium artificial cerebrospinal fluid (aCSF) containing the following (in mM): 125 NaCl, 25 NaHCO3, 2.5 KCl, 1.25 NaH2PO4, 0.5 CaCl2, 4 MgCl25 25 glucose, pH 7.4, oxygenated with 95%O2/5%% CO2 gas.
Similar(53)
Briefly, sections were immersed in fixative (4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4).
Briefly, sections were immersed in boiling 10 mM sodium citrate at pH 6.0 for 3 min in a pressure cooker.
Briefly, tissue sections were immersed in 200 mL of citrate and incubated three times for 5 min in a microwave at 650 W before staining.
Briefly, brain tissues were immersed successively in 4% paraformaldehyde for 6 hours, 15% saccharose in 0.1 M PBS overnight, and then 30% saccharose in 0.1 M PBS overnight.
Briefly, they were hydrated and immersed in 0.01 M citrate buffer (pH 6) in a plastic Coplin jar, which was placed in a microwave oven for 15 min at maximum power (650 W).
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