Sentence examples for immediately upon removal from inspiring English sources

Plants were harvested at 21 days after planting, with aerial shoots and swollen hypocotyls (edible portion of the radish referred to as the "root" hereafter) separated immediately upon removal from the chambers.

Immediately upon removal of the frozen epithelia samples from the −80°C freezer, the lysis buffer from the RNAqueous® Kit (Ambion, Austin, TX) was added.

Furthermore, attempts to refold them by either fast dilution or dialysis in several buffers all failed because recombinant proteins precipitated immediately upon removal of urea, indicating that they were neither refoldable nor insoluble in buffers.

The pain intensity rating (i.e., VAS) was measured immediately after plaster application and immediately upon removal.

Specimens were snap-frozen in liquid nitrogen in the operating theatre immediately upon removal.

Tissue samples were snap-frozen in the operating theatre immediately upon removal and stored in liquid nitrogen until analysis.

This is supported by the observations that (a) AT3.1 cells are closely apposed in free suspension aggregates but separated by matrix in agarose gels, and (b) the adhesion holding together the large spheroids is not immediately reversed upon removal from the gel.

Secondly, it is known that the process of skin closure begins almost immediately upon MN removal, unless the skin area is kept under heavy occlusion.

In addition, a portion of human pancreatic tissue samples was preserved in RNAlater (Ambion Europe Ltd, Huntingdon, Cambridgeshire, UK), or snap-frozen in liquid nitrogen immediately upon surgical removal and maintained at −80°C until use.

The artery samples were upon removal immediately placed in cold Dulbecco's modified Eagle's medium (DMEM, Gibco, Invitrogen, Carlsbad, CA, USA) in Lund and in cold medium M199 (Gibco, Invitrogen, Carlsbad, CA, USA) in Rotterdam and immediately transported to the laboratory where they were prepared in an identical way to the rat MMA.

16 Immediately upon application, and after removal of the plasters (i.e., in 0, 2 and 24 h treatment groups), the VAS, TEWL and any change in skin colour was recorded in all the four interventions, as follows; Pain sensation associated with plasters was determined on a VAS.