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Fluorescent readings were taken immediately (time 0) and every 15 min over 60 min, with the plates maintained under dark conditions and incubated under exposure conditions (37°C/5% CO2) between measurements.
"Secondly, when you break down our 'Red' calls into Red 1s (immediately life-threatening, e.g. cardiac arrest) and Red 2s (serious but less immediately time critical, e.g. suspected stroke), we performed much better on the higher acuity Red 1 calls (57.9%) than the Red 2 calls (40.6%).
One was harvested immediately (time 0) and the remaining parasites returned to culture for harvesting at 6-hour intervals until 42 hours post-invasion.
In Protocol-A, briefly, logarithmically growing HeLa cells were transfected with siRNA specific for Rad21 and 48 hours later, cells were either harvested immediately (time zero) or after a further 2 hours with nocodazole.
Immediately (time t = 0), and then again after a few minutes (2, 4, 6 and 8 minutes for foraging model parameterization; 5 minutes only for evolution experiments), 150 µL of this mixture was centrifuged for one minute at 10K rpm and 100 µL of the supernatant was plated to count the unbound phages.
Wounds were observed and photographed immediately (time 0) and thereafter at 24 and 48 h.
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The Planning Commission removed caps on the number of poor an individual state can have and formally de-linked subsidies from the income threshold, but did not agree to change its definition of poverty, at least immediately (Times of India).
Some 203 individuals completed a series of questionnaire measures immediately pre (time 1) and immediately post (time 2) training during the period March 2004 to February 2005.
Some 203 individuals completed a series of questionnaire measures immediately pre (time 1) and immediately post (time 2) training and there were significant improvements in attitudes and confidence of participants.
Passengers were advised that from 9 31 a.m. to 3 31 p.m., all trains must leave immediately on time.
But Sultana Begum, Oxfam's policy advisor for the Syria crisis, urged donors to release their money immediately this time.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com