Sentence examples for immediately thawed from inspiring English sources

To revive PBMC, cryovials were immediately thawed in a 37°C water-bath, washed twice in 10% RPMI-media and viability was assessed by trypan-blue exclusion.

The samples were then flash-frozen in 96-well cold block (−80°C) for 15 s and then immediately thawed in a 96-well heat block (96°C) for 15 s.

Cryopreserved lymphocytes at the end of each period were taken and immediately thawed at 37°C.

The oocysts were frozen in a dry ice/ethanol bath and immediately thawed in a water bath at 42°C.

In the freeze-thaw cycles, the liposomal solution was frozen in dry ice for approximately 15 minutes and immediately thawed in a 45°C water bath.

Between June 2008 and March 2010, frozen blood samples were sent to the Clinical Chemistry laboratory of the University of Liège where they were immediately thawed and assayed.

Next, cells were subjected to two freeze-thaw cycles by incubating on isopropanol/dry ice bath for 30 seconds and immediately thawing at 37°C.

The freeze thaw method consisted of repeatedly freezing the protein sample at −80°C for 5 min and immediately thawing in a warm water bath at 37°C for 5 min.

In addition to uncertainty over the optimum FFP RBC ratio, there are a number of logistical considerations, such as potential issues with the availability of thawed FFP, the waste that could be created by immediately thawing large quantities of FFP (because of the short shelf-life of thawed FFP) and the need for ABO-compatible plasma early on.

In the present work, the range of post-thaw sDFI values immediately after thawing (6.55-22.46%) recorded is similar to that reported by López-Fernández et al. [ 9] (4.2-26.3%).

However, it should be noted that, in these two studies, the sDFI was analysed immediately after thawing; no post-thaw incubation study was undertaken - and DNA integrity would be most strongly affected during such an incubation period.