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These samples were immediately processed with protein extraction.
Then, 500 μl binding buffer was added and the cells (10 000 cells per sample) were immediately processed with a FACScan flow cytometre (Becton Dickinson, San Jose, CA, USA).
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Additional water samples were filtered on 0.2-µm pore-size polycarbonate filters and immediately processed together with fresh mucilage samples for DNA extraction.
All the elements with a timestamp (tau ) are immediately processed when an element with timestamp greater than (tau ) (and the corresponding watermark) is received, without waiting for out-of-order elements.
All whole blood samples (100 μL) were immediately processed for multiple staining with 5 μL (VioBlue -anti-CD3 and 5 μL (PE)-anti-CD56 antibodies to discriminate NK cell subsets (CD3-/CD56bright and CD3-/CD56dim).
Whole blood (100 μl) was immediately processed for double staining with 20 μl of fluorescein isothiocyanate (FITC -anti-HLA-DR antibody (Beckman Coulter, Marseille, FITC -anti-HLA-DRFITC -anti-HLA-DRtibody (Beckman Coulter) and 4 μl oFITC -anti-HLA-DRPE)-antibody4 antiBeckmanY4-RD1, BeCoulteroulter, Fullerton, CA, USA).
The sample was kept in physiologic saline solution, and immediately processed using a previously described protocol with minor modifications [17].
After incubation, cells were washed with PBS and immediately processed for immunofluorescence analysis.
The perfused liver samples collected were immediately processed, and homogenates (20% w/v) were prepared with ice-cold 1.15% KCl.
Sputum was immediately processed.
Specimens were immediately processed.
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