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The nano-alum mixture was then sonicated immediately prior to application, yielding a semi-clear solution ready for immune studies.
Spore suspensions were vortexed thoroughly immediately prior to application to ensure spores were fully dispersed.
Cells were harvested by centrifugation and transferred to ASW immediately prior to application on T. wilhelma.
The change in fEPSP magnitude was calculated relative to the mean of the 6 data points obtained immediately prior to application of LFS.
For ZnO exposures under submerged conditions, the culture medium in each well was replaced with serum-free medium into which NPs of 0.7 and 2.5 μg/cm of well area were given by adding the appropriate volume of a freshly prepared 1 mg ZnO/mL H2O stock suspension (vortexed and sonicated twice for 1 min intermittently immediately prior to application).
There were no differences in photosynthetic or transpiration rates, stomatal conductance, or internal leaf CO2 concentration between Alethea-treated anon-Aletheahea treated control plants 24 h following Alethea treatment, i.e., immediately prior to application of salinity stress (Net photosynthesis: Alethea = 16.28 ± 0.27 μmol CO2 m-2 s-1, H2O = 15.91 ± 0.30 μmol CO2 m-2 s-1; P > 0.05).
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On the treatment day, vital signs and NPRS "Pain Now" scores were recorded immediately prior to anesthetic application, 30 and 55 minutes after anesthetic application, 25 and 55 minutes after patch application, and 5, 25, 55, and 85 minutes after patch removal.
Transcript(s): A scan of your current official or unofficial transcript; include final grades for the term immediately prior to the application deadline.
Usually you should have been resident in the UK for nine out of the past 10 years immediately prior to your application, unless you have served overseas with HM Forces or in some other official capacity as a representative of Her Majesty's Government, studied abroad or lived overseas with your parents.
In a separate test, all treatment solutions (1 8; Table 1) were prepared as for the penetration experiment but with the addition of a water-soluble fluorescent dye, (Blankophore P, Bayer) at a concentration of 0.5% w/v immediately prior to droplet application.
All of the vines were defoliated in the bunch zone immediately prior to the application of the covers to prevent the leaves from shading the uncovered bunches.
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