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The stages of change include pre-contemplation (not thinking about making changes), contemplation (thinking about making changes, but not immediately), preparation (planning to make changes within the next 30 days, and may already be making small changes), action (initiated the changes in the past 6 months), and maintenance (maintained the changes for more than 6 months) [ 17].
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In our assay, all vesicle preparations were digested immediately after preparation since freezing likely interferes with membrane integrity, making intravesicular protein accessible to trypsin.
Seeds were prepared as described previously [16] and added immediately after preparation.
On the first day, Bogdanov offered them the option of remaining clothed until the dress (for them, undress) rehearsal, or taking off their clothes immediately as preparation.
Spectral measurements were performed immediately after preparation of solutions.
Macroporous or standard cement was injected immediately after preparation of the defect.
Each bottle was sampled immediately after preparation to determine the elemental composition of the aqueous phase.
This was evident because the beads appeared perfectly spherical immediately after preparation.
However, lipids can also be protected from oxidation by addition of antioxidants immediately after preparation [15].
We show that the composite electrolyte is the most amorphous one immediately after preparation and better resists crystallization.
To avoid any possible oxidation of samples, they are isolated in the pumped cryostat immediately after preparation and measured.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com