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An aliquot of the filtered sample was immediately passed through an anionic exchange column that retains As V) but not As III) [21] and, after acidification, analyzed by AAS with a detection limit of 5 μg L-1.
Before each experiment, aliquots of the LC were thawed to room temperature and immediately passed through a PD-10 gel-filtration column equilibrated with Buffer P. The protein was collected in Buffer P and stored on ice.
The assay solution was immediately passed through a 3 kDa cutoff Amicon ultrafilter (Millipore, Billerica, MA).
Lysates were immediately passed through a 10 kDa molecular weight cut-off filter (Biovision, Milpitas, CA, USA).
The eluates were immediately passed through a PD MiniTrap G-25 column (GE Healthcare) equilibrated with PBS.
After 12 h at 37 °C, the cells were removed by centrifugation, and the supernatant solution was immediately passed through a 3 kDa cutoff Amicon ultrafilter (Millipore, Billerica, MA).
Similar(54)
While much of that money will immediately pass through the clubs in wage payments to better players, enough is left over to justify labelling the match the biggest, in financial terms, in the clubs' history.
Both studios, however, immediately passed.
What makes CMBS paper appealing is the pass-through tax status of the entity that manages the loans, known as a real estate mortgage investment conduit (REMIC) The REMIC passively collects interest from the hotel owner and immediately passes it through to CMBS holders.
"They just pass through".
They are just passing through".
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