Put them immediately on ice as cleaned crabs deteriorate rapidly at room temperature, and proceed with your recipe.
Samples were placed immediately on ice and centrifuged at 1000 × g and 4° for 5 min within 1 h, and the plasma was stored at −80 °C until analyses.
Lysates were placed immediately on ice to inhibit proteolysis.
To these parasites, 125 µg/ml of Alexa-conjugated transferrin (Molecular Probes) was added and at different time intervals, aliquots were removed and placed immediately on ice.
BALF samples were placed immediately on ice, filtered with 0.2-µm syringe filters (SFCA Fisher Scientific, Pittsburg, PA), and stored at −80°C for future analysis.
One microlitre of the PCR product was added to 3 µl of Rox 500XL (Applied Biosystems, Warrington, UK) and Elliomut loading buffer (Ellios biotek Laboratory, Paris, France), denatured at 96°C for 2 minutes and cooled immediately on ice.
Aliquots of 25 to 50 ng of sonicated DNA were adjusted to a volume of 10 µl, heat-denatured for 5 min at 100°C, then cooled immediately on ice.
The samples were placed immediately on ice.
Boil for 5 minutes and place immediately on ice.
Treated nucleus pulposus cells were placed immediately on ice and washed with cold PBS.
The samples were incubated at 95°C for 10 min and placed immediately on ice.
