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The 1 mL fractions were captured in Eppendorf tubes that contained 200 μL of 1 M Tris pH 8.8 to immediately neutralize the antibodies.
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The bound phages were retrieved by adding 0.2 M Glycine HCl (pH2.2) containing 0.5 % BSA, and immediately neutralized with 1 M Tris HCl (pH9.1).
The nitrogen ions and silver particles arriving at the exposed silicon substrate were immediately neutralized since the silicon substrate was conductive.
OH− and TPP ions in the acidic CS solution could compete to interact with the -NH3+ of CS, but OH− ions would be immediately neutralized by H+ ions and increase the pH of the CS solution.
After blocking with 0.1% casein in phosphate-buffered saline (PBS) for 2 h and incubation with phage-displayed sdAbs in PBS for 1 h at room temperature, the specific phages were eluted with 100 mM triethylamine, transferred to a fresh tube, and immediately neutralized with 1.0 M Tris-HCl (pH 7.4) and used to infect TG1 cells.
Saturday morning we had our first full bath in a week, which was immediately neutralized when we had to slip back into the same clothes we'd been wearing for the same period.
Immunoprecipitated proteins were eluted in 0.1 M glycine pH 2 and immediately neutralized with 1 M Tris base.
Bound scFv were then eluted with 100 mM triethylamine (Sigma) and immediately neutralized in 1 M Tris-HCl, ph 7.0.
The pH of the elution was immediately neutralized to pH 7.0 using 1 M Tris buffer (pH 10.0).
The eluted antibody was immediately neutralized with 5 M NaOH and 1 M Tris, dialyzed overnight into PBS, concentrated, and supplemented with glycerol (to 50%).
After extensive washes with PBS, antibody was eluted with 200 mM glycine-HCl pH 2.5 and immediately neutralized with 1 M K2HPO4.
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