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Two freezing rates were used: immediately freezing the vials on a pre-cooled (−50°C) freeze-dryer shelf (cooling rate of the sample approximately 2.5°C/min); and freezing at 1°C/min by placing the vial on the shelf at 20°C and cooling the shelf to −50°C at a rate of 1°C/min (cooling rate of the sample approximately 0.8°C/min).
Why not wipe child identity theft off the map by creating and immediately freezing a credit file for every newborn baby?
British authorities reacted immediately, freezing Landsbanki's assets pursuant to anti-terrorist legislation.
By collecting and immediately freezing muscle tissue after DNA injection, Doh et al. demonstrated that luciferase expression could be detected in vitro as early as 30 min post injection (in some mice already after 2 min) [27], showing that even without electroporation, plasmids can be translated within minutes after delivery.
The reactions were stopped by immediately freezing in liquid N2.
We also froze down communities without fixing by directly embedding them in OCT (Piccirillo et al., 2010) and immediately freezing them on dry ice.
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Switzerland immediately froze the assets of the former president.
Tissues obtained from mice were immediately frozen in liquid nitrogen.
The samples were immediately frozen (−70°C).
Blood samples were centrifuged and immediately frozen.
Tumors were removed and immediately frozen in liquid nitrogen.
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CEO of Professional Science Editing for Scientists @ prosciediting.com