The cannula was then immediately flushed with a 10-mL saline solution.
For Contrast Enhanced MR studies (CE-MRI), contrast agent (0.1 mmol/kg, Magnevist, Berlex, Montville, NJ), which corresponds to 50 µL of diluted contrast agent for a 25 g mouse, was injected through a tail-vein catheter [37], [38] and immediately flushed with 25 µL saline.
After loading, the Acrodiscs were immediately flushed with 30 ml of PBS.
The permanent-catheter systems were immediately flushed with 10 ml 0.9% NaCl solution following insertion.
If the aspiration was successful, the catheter lumen was immediately flushed with 10 ml sodium chloride and/or heparin.
Following the harvest, the stomach was immediately flushed with saline and examined under a dissecting microscope (1.8 ×) with a square grid eyepiece.
Rats were placed into a polycarbonate induction chamber, which was closed and immediately flushed with a 5% isoflurane mixture in air for 2 minutes.
A dose of 0.3 mg/kg ICG was given through a cubital fossa vein as a bolus and immediately flushed with 10 ml normal saline.
To render the channels fluorophilic they were immediately flushed with a 0.5% v/v solution of trichloro(1H,1H,2H,2H-perfluorooctyl)silane in Fluorinert FC-40 (3M) and subsequently cured at 120 °C overnight.
The chamber was immediately flushed with a humidified gas mixture (5% CO2, 95% N2) for two minutes at a flowrate of 2.5 l/min to ensure a >99% gas exchange in the chamber which was then sealed.
