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One of the captured Spanish soldiers was immediately decapitated.
At 5, 10, 15, 20, 25 and 30 min after injection, blood was collected from the right carotid artery and the mice (n = 5 for each time point) were immediately decapitated.
Pregnant rats were sacrificed by isoflurane overdose and immediately decapitated to ensure euthanasia before embryo collection.
Briefly, IL-1RI KO and WT mice were killed by cervical dislocation and immediately decapitated.
Mice were immediately decapitated and blood glucose was measured in neck blood.
2 days after the last behavioral test, the animals were stimulated for 2 hr and immediately decapitated with isoflurane anesthesia.
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For killing, individual rats were quickly anaesthetized (<15 sec) in a glass jar containing isoflurane (Merial Animal Health Ltd., UK) vapor, after which animals were decapitated immediately and their whole brains removed, snap frozen in isopentane at −40°C and deep-frozen in dry ice.
Mice were decapitated immediately at the end of the post-wash.
Mice in the WD group were decapitated immediately after the last 48-h WD.
The animals were decapitated immediately after the blood collection and brain was excised at the above mentioned time points.
Rats were decapitated immediately after different time of reperfusion and then the CA1 region of hippocampi were isolated and quickly frozen in liquid nitrogen.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com