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Cell membranes were lysed by heat-shock in a 37 °C water bath and immediately chilled on ice.
The cakes, when first formed, are almost runny, but they are immediately chilled until the butter and cream harden completely.
Cells expressing a particular IMP-GFP fusion were grown until exponential phase, 6 h, as described above, immediately chilled on ice, and analyzed by flow cytometry.
The cultured cells were immediately chilled, collected by centrifugation, and washed with TMA-I buffer (10 mM Tris-HCl (pH 7.8), 30 mM NH4Cl, 10 mM MgCl2, and 6 mM 2-mercaptoethanol).
At Txomin, the grapes are hand-harvested and delivered to the winery, where they are immediately chilled down nearly to freezing and blanketed with nitrogen, an inert gas that prevents oxidation, a process that preserves freshness, juiciness and tangy acidity.
First, 1 μg RNA, 0.5 μl Oligo dT Primer (50 μM), 0.5 μl random hexamer primers (50 μM), 1 μl dNTP mixture (10 mM) and an appropriate volume of RNase-free water up to 10 μl were gently mixed, heated to 65 °C for 5 min; and then immediately chilled on ice.
Similar(9)
Slipstream used to offer cold-brew, but now Brodey prefers to flash-brew his iced coffee — a technique that involves brewing it hot, then immediately chilling it.
Remove with a slotted spoon or strainer and immediately chill in a bowl of ice water or rinse under cold running water to stop the cooking.
At the same time, a lengthy investigation into C.I.A. wrongdoing could immediately chill the relationship between the White House and the spy agency at a time when the C.I.A. is central to the American campaign against terrorism.
If you're not using this immediately, chill it in the refrigerator until needed.
The cold air will immediately chill the t-shirt you have under it, and subsequently turn your wardrobe into a beer cooler.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com