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Pre-OP baseline, before induction of anaesthesia, intra-op immediately before termination of surgery, post-OP 24 h after surgery.
All animals were sacrificed 3 days after the last dose and CSF was collected from the cisterna magna under isoflurane anaesthesia immediately before termination.
All animals were sacrificed 4 days after the last dose and CSF was collected from the cisterna magna of all animals under isoflurane anaesthesia immediately before termination.
In addition to clinical routine measurements, serum samples from patients were drawn directly before induction of anaesthesia (pre-OP), immediately before termination of surgery (intra-OP) and 24 h postoperatively (post-OP).
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Lung ultrasound was performed immediately before SBT.
Similarly, at the termination of locomotion, there was a sustained decrease in EPSC rate from 139 ± 10 Hz to 106 ± 7 Hz (p = 0.0118, paired t-test, n = 23 periods from 9 cells comparing a 500 ms window immediately before and 1 s after locomotion onset).
Do this immediately before hanging.
In support of cycling-off at 70%% of peak EAdi being feasible was our finding that assistance was either EAdi-terminated when flow corresponded to 46%% of peak flow or immediately before EAdi termination due to the inspiratory muscle relaxation increasing pressure in the circuit by 3 cm H2O above the targeted pressure (Servo-I manual).
The reverse primer starts from the codon immediately before the termination codon and carried the Gateway B2.1 sequence at its 5' end.
With gfp inserted immediately before the termination codon or after the start codon of pqn-21 in WRM0622dE07, nuclear-localized GFP was observed in apparently all somatic cells (WBID Expr9751/9754).
When gfp was inserted immediately before the termination codon shared by all three transcripts, reporter expression was observed quite broadly, throughout development in the hypodermis, in body wall muscle in the anterior, in head and ventral cord neurons, in the intestine and in the distal tip cells (WBID Expr9742).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com