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Surgically-resected tissues were snap-frozen on dry ice immediately after resection and stored at -80°C until use.
Fresh human GC tissues were obtained from patients immediately after resection.
Immediately after resection, tumour specimens were examined using confocal microscopy (Leica Microsystems Ltd) as previously described [20].
Specimens were snap-frozen immediately after resection.
Tumor tissue was sampled immediately after resection of the specimen and instantly frozen in liquid nitrogen.
Specimens were obtained immediately after resection and snap-frozen until use.
Tissues of interest were snap-frozen in liquid nitrogen immediately after resection and stored at −80°C.
Immediately after resection, the tissues were snap-frozen in liquid nitrogen and stored in a local biobank at −80°C until RNA isolation.
We have analyzed the functional state of human TIL immediately after resection of three types of tumors (NSCLC, melanoma and RCC).
Three matched patient biopsies were taken immediately after resection.
Samples were flash frozen in liquid nitrogen immediately after resection.
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